Cucurbitane Glycosides from Siraitia Grosvenorii and Their Hepatoprotective Activities

<i>Siraitia grosvenorii</i> (<i>S. grosvenorii</i>), a traditional medicine food homology plant, serves both dietary and medicinal purposes and is increasingly exploited for its bioactivities in pharmaceuticals and nutritional value. In this research, fifteen glycosides including three new cucurbitane-type triterpenoid glycosides named Luohanguosides A-C (<b>1</b>-<b>3</b>) and twelve known ones (<b>4</b>-<b>15</b>) have been isolated from the aqueous extract of fresh <i>S. grosvenorii</i> fruits. A comprehensive analysis of 1D, 2D-NMR, HRESIMS techniques along with some other spectroscopic methods led to the elucidation of their chemical structures. Further investigation focused on the hepatoprotective activities of compounds <b>1</b>-<b>15</b>. It turned out that compounds <b>1</b>, <b>5,</b> and <b>10</b> exhibited significant hepatoprotective activities compared to bicyclol under the same concentration (20 μM), providing scientific support for further research on <i>S.grosvenorii</i> products for their preventive potential of hepatic diseases.