Evaluation of Antioxidant, Anticholinesterase and Antiproliferative Activities of the Aerial Parts of Dryopteris raddeana

This study aimed to evaluate the biological activities of the ethanol extract obtained from the aerial parts of Dryopteris raddeana (Fomin) Fomin. Total antioxidant capacity (TAS), total oxidant level (TOS) and oxidative stress index (OSI) values ​​determined by Rel Assay kits using ethanol extract obtained from aerial parts of the plant were determined as 5.099±0.076 mmol/L, 7.354±0.107 µmol/L and 0.144±0.004, respectively. These results show that D. raddeana exhibits a balanced oxidative profile and has low oxidant load. In anticholinesterase activity analyses, the inhibitory effect of the extract against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes was determined as 58.99±1.48 µg/mL and 83.51±1.59 µg/mL, respectively. In the evaluation of antiproliferative effect, it was observed that extract concentrations in the range of 25–200 µg/mL applied to A549 human lung cancer cell line decreased cell viability in a dose-dependent manner. A significant decrease in cell viability was detected especially at concentrations of 100 and 200 µg/mL. In conclusion, in this study, it was determined that the plant could be an antioxidant, antimicrobial and antiproliferative source for these biological activities of D. raddeana.