Mutation v1

Induce the mutator DNAP with rhamnose to drive mutations on the linear O-replicon; after the predetermined evolution time/generations, remove rhamnose to shut off mutagenesis and switch to “normal replication” to stabilize the mutations for downstream analysis.

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Publication Details

Published
2025-10-03
DOI
https://doi.org/10.17504/protocols.io.4r3l21yeqg1y/v1
Primary Topic
DNA Repair Mechanisms
Type
article
Field-Weighted Citation Impact
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article

Mutation v1

igemnthuth not provided
DNA Repair Mechanisms
article

Mutation v1

igemnthuth not provided
article en

Abstract

Induce the mutator DNAP with rhamnose to drive mutations on the linear O-replicon; after the predetermined evolution time/generations, remove rhamnose to shut off mutagenesis and switch to “normal replication” to stabilize the mutations for downstream analysis.

Openalex Percentile: Top 75%
DNA Repair Mechanisms
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Mutation v1 — igemnthuth not provided · (2025) | TGRS Research Map | TGRS