Comparison of impact and duration difference from conventional cigarette smoke and electronic cigarette smoke to paneth cell metaplasia and goblet cell hyperplasia in Rattus norvegicus colon in vivo

Introduction: Paneth cells contribute as a sign of change in colon biota, where the cells are forced to become metaplastic to survive in the environment, while goblet cells become the sign of increased inflammation, causing them to become hyperplastic. The purpose of this experiment is to compare the type and difference in duration of smoke to Paneth cell metaplasia and goblet cell hyperplasia. Method: This experiment is an analytic study with an experimental design to do a direct intervention to get the correlation between variables. Thirty-five Rattus norvegicus, a Sprague Dawley strain, is used because it is not aggressive and easy to control. All of the data from this experiment will be analyzed using Two-Way ANOVA to determine the correlation between two categorical variables. Result: Thirty-five Rattus norvegicus were included in this experiment. Goblet cell hyperplasia can be found significant based on week 2 and week 4 on both cigarette types (p<0,05). Goblet cell hyperplasia was found insignificant based on both cigarette types (p>0,05). On the other hand, Paneth cell metaplasia is not found in both cigarette type and duration. Conclusion: There is no correlation between the type of cigarette on goblet cell hyperplasia. There is a significant correlation between differences in exposure duration on goblet cell hyperplasia. Paneth cell metaplasia is not found on both week 2 and week 4 on conventional and electronic types of cigarettes.