The effect of a biostimulator on the root formation of poplar cuttings Pyramidal-sedge Kamyshinsky

The problem of growing high-quality tree planting material is solved by using biologically active substances. Most poplars, including the Pyramid-sedge Kamyshinsky, are propagated by cuttings. But the weak root system of the pyramid-shaped poplars makes them vulnerable to drought, winds and mechanical damage. This paper presents the results of obtaining a biostimulator by fermenting collagen raw materials with actinomycete Streptomyces fradiae AC – 570 and evaluating its effect on the formation of adventitious roots in cuttings of the Pyramidal-sedge Kamyshinsky poplar. The amino acid composition of collagen hydrolysate was determined using liquid chromatography on a Shi-madzu LC-20 Prominence apparatus (Shimadzu, Japan). The degree of hydrolysis of collagen raw materials was as-sessed by amine nitrogen, using the Sorensen method. It was found that the optimal conditions for obtaining a bi-ostimulator are autoclaving of crushed raw materials with a 1:6 hydromodule for 20 minutes at a pressure of 0.1Mpa, followed by fermentation for 36 hours at a temperature of 28-30 0C, pH 6.5 – 7.0 and a concentration of culture of the microorganism of 10%. Processing of collagen raw materials under these conditions increased the degree of hydrol-ysis by 5.9 times. When observing these modes of collagen biodegradation, a high content of amino acids is provided, which allows it to be used as an amino acid stimulator of plants. The content of proline increased by 64.6%, and gly-cine by 7.5 times. Treatment of cuttings with a biostimulator increased the root mass by more than 3 times, and the leaf biomass of plants by 36.8%. The total weight of the cuttings of the prototypes exceeded the control variants by 1.9 times. The data obtained can be used to enhance the root formation of the cuttings of the Pyramid-sedge Ka-myshinsky poplar.