232 Late-Breaking: Breed-specific lipid profiles in cryopreserved goat sperm.

Abstract This research presents a comparative investigation of the lipidomic profiles of sperm from goat breeds of the Alpine and Spanish goat breeds. The purpose of this study is to investigate breed-specific changes in fatty acid profiles and their consequences for sperm function and reproductive effectiveness. Following the extraction of lipids from cryopreserved semen samples of mature bucks of the Alpine (n=6) and Spanish (n=4) breeds, the samples were then examined by gas chromatography-mass spectrometry (GC-MS). The results of this analysis revealed that the two breeds included a total of 21 different fatty acids. There were eleven fatty acids that exhibited statistically significant changes (p < 0.05). These fatty acids included 13:0, 16:0, 18:0, 20:0, 24:0, 14:1, 18:1 (cis-9), 24:1, 18:1 (trans-9), 18:2, and 22:6. In the Alpine breed, the levels of 16:0, 18:0, 20:0, 24:0, 18:1 (cis-9), 18:1 (trans-9), 18:2, and 22:6 were found to be greater. On the other hand, the levels of 13:0, 14:1, and 24:1 were found to be higher in the Spanish breed (p < 0.05). Based on the statistical analysis, it was determined that 16:0, 18:1 (cis-9), and 18:2 were statistically and physiologically significant (p < 0.05, FC > 2). Furthermore, the concentrations of total fatty acids, total saturated fatty acids (Total-SFA), total-trans-monounsaturated fatty acids (Total Trans-MUFA), and total polyunsaturated fatty acids (PUFA) were found to be significantly higher in the Alpine breed. On the other hand, the concentrations of total cis-monounsaturated fatty acids (Total cis-MUFA) were found to be significantly higher in the Spanish breed (p < 0.05). 16:0, 18:1 (cis-9), and 18:2 were shown to have contributed to the majority of the central nodes of the lipidomic network, which may be responsible for membrane stability and cryotolerance, according to the findings of network and pathway studies. The lipidomic variances that have been discovered between breeds may be related to both genetic and environmental causes. These differences may provide significant tools for improving breeding strategies, artificial insemination programs, and cryopreservation techniques for sperm.