PSIII-23 The Effects of exposure to Bisphenol A during pregnancy on the blood and uterus of mice.

Abstract Bisphenol A (BPA) is a raw material that has been widely used in the manufacture of plastics and cooking tools. However, it has been proven to be reproductively toxic. In order to investigate the effects of exposure to BPA on the uterus and blood BPA concentration of mice during pregnancy, forty-eight 8-month-old specific pathogen-free (SPF) pregnant mice with similar body weight (18-22 g) were randomly divided into two groups with the same diet (Control group and BPA group). The control group had 24 mice, and all the mice were fed with water at the beginning of the pregnancy (day 0) until 18 days. The BPA group had 24 mice, and all the mice were fed with water + 50mg/kg BPA at the beginning of the pregnancy (day 0) until 18 days. All mice from the two groups were sacrificed on days 3, 6, 9, 12, 15, and 18. In the meantime, blood samples were collected to determine serum BPA levels, and the uterus samples were collected to measure the number of embryos and immune health conditions. The results show that the concentration of BPA in the serum of pregnant mice of the BPA group at each time point (day 3 to day 18) is significantly higher than the Control group (P< 0.05). The number of embryos of mice in the BPA group is significantly lower than the number in the Control group on day 18 (P< 0.05). For the immune health conditions, the results show that the ratio of CD4/CD8, an indicator of the balance and overall health, in the uterus of the Control group on days 9 and 12 is significantly higher than the BPA group (P< 0.05). The results indicate that exposure to BPA during pregnancy via water could cause the abortion and affect the health condition of the uterus of mice.