308 Influence of spray-dried plasma on fecal metabolites, gut microbiota, and inflammatory biomarkers in canine nutrition.

Abstract Spray-dried plasma (SDP) is a functional protein ingredient derived from animal blood. It is recognized for its high nutritional value and bioactive compounds, including immunoglobulins, growth factors, and biologically active peptides. However, its effects on companion animal nutrition, particularly in extruded canine diets, have not been extensively explored. The aim of this study was to evaluate the effects of SDP inclusion in extruded canine diets on fecal fermentative metabolites, fecal microbiota composition, and inflammatory biomarkers. Twelve adult Beagles were assigned to 4 dietary treatments in a replicated 4×4 Latin Square design: a control diet (0% SDP), 4% SDP applied as a kibble coating, and 4% or 8% SDP incorporated pre-extrusion. Dogs consuming 8% SDP had higher fecal concentrations of short-chain fatty acids, particularly propionate, and lower levels of protein fermentation byproducts, such as 3-methylindole (P < 0.05). SDP inclusion also modulated fecal microbiota composition, increasing the relative abundance of Lactobacillus in dogs fed the 4% Coating SDP diet and Catenibacterium in those receiving 4% SDP (P < 0.05). Additionally, higher fecal IgA concentrations (P < 0.05) were observed in dogs fed the 8% SDP, suggesting a significant enhancement of mucosal immunity, possibly contributing to improved intestinal homeostasis and overall immune defense. Overall, the findings of this study support SDP’s potential as a functional ingredient in canine diets, promoting gut health.