PSVI-14 Inhibition of Escherichia coli growth by a unique essential oil blend: A potential natural antimicrobial strategy.

Abstract The increasing prevalence of antibiotic-resistant bacteria has driven the search for alternative antimicrobial solutions, particularly natural compounds such as essential oils. This study evaluated the antibacterial properties of an essential oil blend against Escherichia coli using the broth microdilution method. To determine its effectiveness, minimum inhibitory concentrations (MICs) and 24-hour growth assays were conducted across a concentration range of 0.1% to 50%. The findings demonstrated a concentration-dependent inhibitory effect, with the MIC established at 25%. Growth curve analysis indicated that lower concentrations (0.1% to 0.78%) allowed normal bacterial growth, while intermediate concentrations (1.56% to 3.13%) resulted in irregular growth patterns. At higher concentrations (25% and 50%), bacterial growth was completely inhibited, as reflected by flat growth curves. These results highlight the potential of the essential oil blend as a natural antimicrobial agent and a promising alternative to conventional antibiotics for addressing antibiotic-resistant bacterial infections.