Generation of Nano‐Antibodies Targeting the SNMP1 Membrane Receptor of <i>Spodoptera littoralis</i> Using Extracellular Vesicles

, a key player in transferring hydrophobic pheromones from pheromone-binding proteins to olfactory receptors, thereby triggering behavioral responses in moths. Targeting this protein with Nabs could provide a potential biocontrol strategy by limiting moth reproduction. Llama immunization was carried out using EVs displaying SNMP1 on their surface. A multi-layer approach was then employed, incorporating an anchor protein co-displayed with the target protein to specifically expose the EVs of interest for biopanning through phage display and selection via ELISA. Nabs against SNMP1 were successfully isolated, and their interactions with the membrane protein were characterized using bio-layer interferometry (BLI) with the same multi-layer approach. Furthermore, epitope binning by BLI aligned with predictions made by AlphaFold3.

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Publication Details

Journal
Journal of Extracellular Biology
Published
2026-04-01
DOI
https://doi.org/10.1002/jex2.70118
Primary Topic
Lubricants and Their Additives
Type
article
Field-Weighted Citation Impact
0.00

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article

Generation of Nano‐Antibodies Targeting the SNMP1 Membrane Receptor of Spodoptera littoralis Using Extracellular Vesicles

Laura Fradale, Christian Cambillau, Josiane Kassis-Sahyoun, Alexis Dogliani et al.
Journal of Extracellular Biology
Lubricants and Their Additives
article

Generation of Nano‐Antibodies Targeting the SNMP1 Membrane Receptor of Spodoptera littoralis Using Extracellular Vesicles

Laura Fradale, Christian Cambillau, Josiane Kassis-Sahyoun, Alexis Dogliani, Emmanuelle Jacquin‐Joly, Alain Roussel, Valeille Céline
article en

Abstract

, a key player in transferring hydrophobic pheromones from pheromone-binding proteins to olfactory receptors, thereby triggering behavioral responses in moths. Targeting this protein with Nabs could provide a potential biocontrol strategy by limiting moth reproduction. Llama immunization was carried out using EVs displaying SNMP1 on their surface. A multi-layer approach was then employed, incorporating an anchor protein co-displayed with the target protein to specifically expose the EVs of interest for biopanning through phage display and selection via ELISA. Nabs against SNMP1 were successfully isolated, and their interactions with the membrane protein were characterized using bio-layer interferometry (BLI) with the same multi-layer approach. Furthermore, epitope binning by BLI aligned with predictions made by AlphaFold3.

Journal of Extracellular BiologyVol. 5(4)
Centre National de la Recherche Scientifique (FR), Université Paris-Est Créteil (FR), Université Paris Cité (FR), Sorbonne Université (FR), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), Laboratoire d’Informatique et Systèmes (FR), Institut d'écologie et des sciences de l'environnement de Paris (FR), Institut de Recherche pour le Développement (FR)
Agence Nationale de la Recherche, French Infrastructure for Integrated Structural Biology
Openalex Percentile: Top 100%
Lubricants and Their Additives
DOIOpen Access PDFOpenAlex
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Generation of Nano‐Antibodies Targeting the SNMP1 Membrane Receptor of <i>Spodoptera littoralis</i> Using Extracellular Vesicles — Laura Fradale, Christian Cambillau, et al. · Journal of Extracellular Biology (2026) | TGRS Research Map | TGRS