Stop Evolution v1

Terminate the rhamnose-induced high-mutation-rate evolution phase by replacing the culture medium to remove the inducer, thereby shutting down expression of the mutator DNA polymerase in the OrthoRep system. This allows stabilization and selection of target mutant strains for subsequent phenotypic analysis.

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Publication Details

Published
2025-10-03
DOI
https://doi.org/10.17504/protocols.io.n92ld6q2og5b/v1
Primary Topic
Evolution and Genetic Dynamics
Type
article
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article

Stop Evolution v1

igemnthuth not provided
Evolution and Genetic Dynamics
article

Stop Evolution v1

igemnthuth not provided
article en

Abstract

Terminate the rhamnose-induced high-mutation-rate evolution phase by replacing the culture medium to remove the inducer, thereby shutting down expression of the mutator DNA polymerase in the OrthoRep system. This allows stabilization and selection of target mutant strains for subsequent phenotypic analysis.

Openalex Percentile: Top 70%
Evolution and Genetic Dynamics
DOIOpen Access PDFOpenAlex
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Stop Evolution v1 — igemnthuth not provided · (2025) | TGRS Research Map | TGRS