Add Arabinose v1

To control expression of the synthetic replication operon in the orthogonal replication system, this step uses anarabinose-induced dCas9–sgRNA repression systemto reduce downstream operon component expression, therebyimproving orthogonal replicon transformation efficiency.

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Publication Details

Published
2025-10-03
DOI
https://doi.org/10.17504/protocols.io.dm6gpm558gzp/v1
Primary Topic
Garlic and Onion Studies
Type
article
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article

Add Arabinose v1

igemnthuth not provided
Garlic and Onion Studies
article

Add Arabinose v1

igemnthuth not provided
article en

Abstract

To control expression of the synthetic replication operon in the orthogonal replication system, this step uses anarabinose-induced dCas9–sgRNA repression systemto reduce downstream operon component expression, therebyimproving orthogonal replicon transformation efficiency.

Openalex Percentile: Top 80%
Garlic and Onion Studies
DOIOpen Access PDFOpenAlex
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Add Arabinose v1 — igemnthuth not provided · (2025) | TGRS Research Map | TGRS