Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture
Homemade weekend-free media can provide a cost-effective alternative to commercial formulations. hE8, integrating some features of B8 while resembling cE8, emerges as a robust and practical option with limited compromises. B8+, though advantageous in some contexts, warrants caution due to lineage priming effects that may impact differentiation outcomes.
Authors
- Lukasz Truszkowski (ORCID: https://orcid.org/0000-0001-7816-6092)
- Silvia Becca (ORCID: https://orcid.org/0009-0009-4918-1133)
- Andrea Ditadi (ORCID: https://orcid.org/0000-0003-3283-8943)
- Elisa Balmas (ORCID: https://orcid.org/0000-0002-4600-6809)
- Kirsten E Snijders (ORCID: https://orcid.org/0000-0002-9683-6528)
- Alessandro Bertero (ORCID: https://orcid.org/0000-0002-4919-9087)
- Francesco Neri (ORCID: https://orcid.org/0000-0003-3903-1974)
- Savatore Oliviero
- Sveva Bottini
- Federica Sozza
- Giulia Savoré (ORCID: https://orcid.org/0009-0002-1940-4024)
- Giulia Ferrari Ramondo
- Mirko Giuseppe Scrivano
- Catherine Elton
- Cristina Rubinetto
- Sara Bianchi
- Helen Bell
- Luana Ferrara
- Irene Ricca (ORCID: https://orcid.org/0009-0009-5276-2035)
- Linda Belluci
Institutions
- Cambridge Consultants (United Kingdom) (GB)
- The San Raffaele Telethon Institute for Gene Therapy (IT)
- Istituti di Ricovero e Cura a Carattere Scientifico (IT)
- Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele (IT)
- University of Turin (IT)
Publication Details
- Journal
- Open Research Europe
- Published
- 2025-09-19
- DOI
- https://doi.org/10.12688/openreseurope.18245.2
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00