Comprehensive dissection of rectal cancer organoids in responses to chemoradiation
The responses of cancer organoids to treatments in vitro can predict the clinical outcomes of corresponding patients. Here, we further uncover that the responses of cancer organoids to combined treatments predict the clinical outcomes of locally advanced rectal cancer (LARC) patients more faithfully than monotherapy with 92.50% and 93.75% accuracy in the discovery and validation cohorts, respectively, which is partly due to the synergistic (3.91%, 5/128) and antagonistic (4.69%, 6/128) treatment interactions in combined chemoradiation. In addition, our detailed analysis reveals that the time point for collecting survival data of organoids is critical to the efficacy of organoids' responses to treatments for predicting the clinical outcomes of LARC patients. Besides, our current data favor the addition of irinotecan for neoadjuvant treatment of LARC patients. The comprehensive dissection of rectal cancer organoids in responses to chemoradiation here may have significant implications for the broad application of organoids in cancer precision treatments.
Authors
- Peiyuan Tang (ORCID: https://orcid.org/0000-0001-5247-9058)
- Guichao Li (ORCID: https://orcid.org/0000-0003-4911-5995)
- Yuanchuang Li
- Guoqiang Hua (ORCID: https://orcid.org/0000-0001-9274-8136)
- Liping Liang (ORCID: https://orcid.org/0000-0001-8113-1931)
- Xinxin Rao (ORCID: https://orcid.org/0000-0002-8882-501X)
- Junjie Peng (ORCID: https://orcid.org/0000-0002-0371-4960)
- Jianjun Gao (ORCID: https://orcid.org/0000-0001-6138-4472)
- Guoxiang Fu (ORCID: https://orcid.org/0000-0001-6949-828X)
- Qiang Guo (ORCID: https://orcid.org/0009-0002-0644-350X)
- Ye Yao (ORCID: https://orcid.org/0000-0001-6240-9539)
- Juefeng Wan (ORCID: https://orcid.org/0000-0001-5361-1663)
- Hui Zhang (ORCID: https://orcid.org/0000-0001-8803-2696)
- Min Chen (ORCID: https://orcid.org/0000-0003-4921-0175)
- Jing Zhang (ORCID: https://orcid.org/0000-0002-8449-6554)
- Yaqi Wang (ORCID: https://orcid.org/0000-0002-8003-6251)
- Zhen Zhang (ORCID: https://orcid.org/0000-0001-7825-2679)
- Xiaomeng Li (ORCID: https://orcid.org/0000-0002-7811-3457)
- Sanjun Cai (ORCID: https://orcid.org/0000-0002-2833-9204)
- Lijun Shen (ORCID: https://orcid.org/0000-0001-9459-8338)
- Tao� Lv (ORCID: https://orcid.org/0009-0003-7446-8191)
- Yi Zhou (ORCID: https://orcid.org/0000-0001-5195-5294)
- Xiaoya Xu (ORCID: https://orcid.org/0000-0002-2764-256X)
- Fan Xia (ORCID: https://orcid.org/0000-0001-8185-8663)
- Xiaoxue Gao (ORCID: https://orcid.org/0000-0003-1920-5228)
- Yun Deng
- Mengxue Pan
Institutions
- Shanghai Medical College of Fudan University (CN)
- Fudan University Shanghai Cancer Center (CN)
- Institute of Applied Technology (CN)
Publication Details
- Journal
- Cell Reports Medicine
- Published
- 2025-10-01
- DOI
- https://doi.org/10.1016/j.xcrm.2025.102397
- Citations
- 2
- Primary Topic
- Colorectal Cancer Surgical Treatments
- Type
- article
- Field-Weighted Citation Impact
- 2.09
Funders
- Shanghai Anti-Cancer Association
- National Natural Science Foundation of China
- Langley Research Center