P02.08.A A SPATIALLY RESOLVED HUMAN GLIOBLASTOMA ATLAS REVEALS DISTINCT CELLULAR AND MOLECULAR PATTERNS OF ANATOMICAL NICHES

Abstract BACKGROUND Glioblastoma (GBM) is the most lethal brain tumor in adults with remarkable cellular plasticity and complex anatomical features. While single-cell RNA sequencing (scRNA-seq) has revealed its cellular heterogeneity, the spatial cellular organization within the anatomical features remains elusive. MATERIAL AND METHODS Here, we provide a comprehensive spatial transcriptomic dataset comprising 115,914 transcriptomes from 32 genotyped tissue sections. RESULTS Leveraging Xenium in situ technology, we generated spatial transcriptomic data with subcellular-resolution, significantly improving the granularity of our understanding of GBM cellular architecture. In addition, our analyses identified oligodendrocyte subtypes specifically localized to tumor cores and perivascular regions with molecular profiles associated with poor prognosis. CONCLUSION This publicly accessible GBM atlas provides a valuable resource for understanding the complex cellular architecture of GBM and facilitating the development of novel therapeutic strategies.