Novel Dual-Action Whitening Peptides Derived from Tea Protein Hydrolysates
Despite the known benefits of tea (<i>Camellia sinensis</i>), the functional potential of tea-derived proteins remains largely unexplored. To investigate this, tea proteins were extracted and enzymatically hydrolyzed with alkaline protease to obtain tea protein hydrolysates (TPH). Characterized by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), TPH were screened via integrated <i>in silico</i> and molecular docking for dual-action peptides targeting tyrosinase inhibition and antioxidant activity. Two peptides, EGFG and FGDPHG, were identified and validated as potential tyrosinase inhibitors and antioxidants, exhibiting strong free radical scavenging and iron-chelating capabilities in cell-free systems. In B16-F10 melanoma cells, both peptides significantly reduced α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis and protected against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced oxidative stress. Their whitening efficacy was further confirmed in a zebrafish model. These findings establish EGFG and FGDPHG as novel, dual-action whitening peptides, highlighting their potential as functional ingredients in cosmetics and nutraceuticals.
Authors
- Wan‐Yang Sun (ORCID: https://orcid.org/0000-0002-4439-967X)
- Yi‐Ning Chen (ORCID: https://orcid.org/0000-0003-4821-8511)
- Yi‐Fang Li (ORCID: https://orcid.org/0000-0002-3356-3702)
- Rong‐Rong He (ORCID: https://orcid.org/0000-0001-5505-5672)
- Chang‐Yu Yan (ORCID: https://orcid.org/0000-0001-8530-5484)
- Jianwen Ye (ORCID: https://orcid.org/0000-0003-2562-7283)
- Si Tan (ORCID: https://orcid.org/0000-0003-4409-593X)
- Jing‐Hao Wang
- Hai-Biao Gong
- Weixi Li (ORCID: https://orcid.org/0000-0002-6614-9508)
- Ye Yue (ORCID: https://orcid.org/0000-0001-6835-6722)
- Qi Wang (ORCID: https://orcid.org/0000-0002-5441-9320)
- Yufei Wang (ORCID: https://orcid.org/0000-0002-9667-2105)
- Shisheng Chen (ORCID: https://orcid.org/0000-0002-8617-4356)
- Kun Li (ORCID: https://orcid.org/0000-0002-6778-2233)
- Hiroshi Kurihara
- Xiao-Yuan Gu
Institutions
- Shenyang Pharmaceutical University (CN)
- Second Hospital of Shandong University (CN)
- First Affiliated Hospital of Guangdong Pharmaceutical University (CN)
- Ministry of Education (TH)
- First Affiliated Hospital of Chinese PLA General Hospital (CN)
- Yangtze River Pharmaceutical Group (China) (CN)
- Kai Biotech (South Korea) (KR)
- Second Affiliated Hospital of Shandong University of TCM (CN)
- Yunnan University of Traditional Chinese Medicine (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2025-10-03
- DOI
- https://doi.org/10.1021/acs.jafc.5c05643
- Primary Topic
- melanin and skin pigmentation
- Type
- article
- Field-Weighted Citation Impact
- 0.00