Immune-related hub genes in intrauterine adhesions: a bioinformatics approach

We identified seven immune-related gene signatures through integrative bioinformatics. We experimentally validated three genes (<i>TUBB3</i>, <i>PTGDS</i>, and <i>CCL14</i>) demonstrating species-specific expression patterns. We proposed four mechanistically plausible biomarkers (WNT5A, IGF1, BIRC5, and GDF7) for developing IUA diagnostic tools. The conserved dysregulation of WNT5A- and TGF-β-associated genes (<i>GDF7</i> and <i>IGF1</i>) suggest therapeutic targets for preventing adhesion recurrence. This study advances our understanding of IUA pathogenesis. Single-cell transcriptomics should be examined in future studies to determine the cellular-subtype-specific contributions to IUA.