DNA/RNA extraction and cleanup protocol for PCR and tag seq applications with coral samples v1

The extraction of high-quality genomic material from corals can be difficult due to high concentrations of lipids and carbohydrates in coral tissues. This is especially the case for some corals in the genus Porites. This protocol uses a modified PCA protocol to extract combined DNA and RNA from coral tissues stored in 100% ethanol and frozen. The DNA can be diluted and used in varied PCR applications. If the end goal is RNA sequencing, the RNA cleanup protocol can be followed to acquire clean high-quality RNA.

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Publication Details

Published
2025-10-01
DOI
https://doi.org/10.17504/protocols.io.261gek93jg47/v1
Primary Topic
Coral and Marine Ecosystems Studies
Type
article
Field-Weighted Citation Impact
0.00

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article

DNA/RNA extraction and cleanup protocol for PCR and tag seq applications with coral samples v1

Carsten G. B. Grupstra, Ally Swank, Sarah W. Davies
Coral and Marine Ecosystems Studies
article

DNA/RNA extraction and cleanup protocol for PCR and tag seq applications with coral samples v1

Carsten G. B. Grupstra, Ally Swank, Sarah W. Davies
article en

Abstract

The extraction of high-quality genomic material from corals can be difficult due to high concentrations of lipids and carbohydrates in coral tissues. This is especially the case for some corals in the genus Porites. This protocol uses a modified PCA protocol to extract combined DNA and RNA from coral tissues stored in 100% ethanol and frozen. The DNA can be diluted and used in varied PCR applications. If the end goal is RNA sequencing, the RNA cleanup protocol can be followed to acquire clean high-quality RNA.

Atlantic University (US), Florida Atlantic University (US)
National Science Foundation
Openalex Percentile: Top 73%
Coral and Marine Ecosystems Studies
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