DNA/RNA extraction and cleanup protocol for PCR and tag seq applications with coral samples v1
The extraction of high-quality genomic material from corals can be difficult due to high concentrations of lipids and carbohydrates in coral tissues. This is especially the case for some corals in the genus Porites. This protocol uses a modified PCA protocol to extract combined DNA and RNA from coral tissues stored in 100% ethanol and frozen. The DNA can be diluted and used in varied PCR applications. If the end goal is RNA sequencing, the RNA cleanup protocol can be followed to acquire clean high-quality RNA.
Authors
- Carsten G. B. Grupstra (ORCID: https://orcid.org/0000-0001-5083-4570)
- Ally Swank (ORCID: https://orcid.org/0000-0003-3318-2376)
- Sarah W. Davies (ORCID: https://orcid.org/0000-0002-1620-2278)
Institutions
- Atlantic University (US)
- Florida Atlantic University (US)
Publication Details
- Published
- 2025-10-01
- DOI
- https://doi.org/10.17504/protocols.io.261gek93jg47/v1
- Primary Topic
- Coral and Marine Ecosystems Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation