Construction of protein capsules from novel amphiphilic two-helix bundle proteins (GP-ZiPs), designed by fusing the transmembrane region of glycophorin A with coiled-coil peptides

To further validate our protein capsule construction strategy based on amphiphilic two-helix-bundled proteins, we designed GP-ZIPs (GP-ZIP41, GP-ZIP29, GP-ZIP20), combining a truncated glycophorin A transmembrane region and a dimeric coiled-coil. Using an emulsion method, we successfully constructed stable hollow capsules (70-200 nm) capable of encapsulating hundreds of protein molecules.