Genotyping of vitamin D receptor (foki and BSML) gene polymorphism in male patients with coronary artery disease in diabetic verses non diabetic

The present study aims to correlate the Vit.D levels and frequency of the VDR gene polymorphisms FokI (rs2228570), BsmI (rs1544410) in male patients with coronary artery disease with and without Type 2 diabetes.40 healthy controls, 40 individual experiencing T2DM, 55 patients with CAD and 55 patients with both T2DM and CAD made comprised the 4 groups of participants in this case control study which include totally 190 male individuals.This investigation was conducted during the period from February 2022 to November 2022 at the catheterization unit in cardiologic clinics of Iraqi Center for Heart Disease and specialized center for endocrinology and diabetes, Baghdad Teaching Hospital/ Medical City.Diagnosis depend on physical examination and complete medical history.Two-gene polymorphism were assessed in the current study, 1st one was FOKI in which 3 genotypes were detected in participations.45.8% of all participant had CC genotype, 33.2% had CT and 21.1% had TT.Regarding each group, in DM group 65% had CC, 30% had CT and 5% had TT, the odd ratio and p-value were 0.89 (0.35-2.26), 0.81; 1.28 (0.48-3.44), 0.61; and 0.64 (0.10-4.11), 0.64, respectively.Regarding the group of patients with CAD only, 32.7% had CC genotype, 40% had CT and 27.3% had TT, patients with CAD had 4.62 (1.23-17.27)times higher than control to have TT genotype, p-value 0.02, respectively.The 2nd polymorphism was BSMI, 3 genotype were detected 28.4% had AA, 36.3% had AG and 35.3% had GG genotype, with statistical significant association between the studied group (CAD group and CAD with DM) with development of GG genotype, p-value <0.001.Regarding DM group 40% had AA, 55% had AG and 5% had GG, DM patients had 3.22 (1.26-8.18)times of getting AG in compare to control, p-value 0.01.Regarding CAD group, 10.9% had AA, 38.2% had AG and 50.9% had GG genotype,) times higher chance of getting GG in compare to control, p-value 0.0004.