Low‐Input Assay for Transposase‐Accessible Chromatin Identifies Epigenetic Signatures of Liver Group 1 Innate Lymphoid Cells
Assessing chromatin accessibility in rare cell populations within tissue remains a key challenge. To address this, we present a low-input ATAC workflow optimized for liver ILCs. The protocol is validated across cell numbers, Tn5 ratios, and library preparation steps and unveils unique epigenetic features of liver NK cells and ILC1s.
Authors
- Gabriela M. Wiedemann (ORCID: https://orcid.org/0000-0001-6138-9791)
- Robin Schenk (ORCID: https://orcid.org/0000-0001-6823-8128)
- Kevin Schmid
Institutions
- Institute of Clinical Cancer Research (DE)
- Tumkur University (IN)
Publication Details
- Journal
- European Journal of Immunology
- Published
- 2025-10-01
- DOI
- https://doi.org/10.1002/eji.70066
- Citations
- 1
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 0.80
Funders
- Deutsche Forschungsgemeinschaft