Establishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donor
Pluripotent stem cell (PSC)-based technologies offer a promising new approach to generating functional cells or grafts for transplantation. To minimize the risk of immune rejection, induced pluripotent stem cells (iPSCs) that are homozygous for HLA Class I and Class II genes are a potential solution to reduce the risk of immune rejection in regenerative medicine applications. In this study, MUSIi023-A iPSC line was generated from peripheral blood mononuclear cells of a Thai donor with 11 loci homozygous HLA. This cell line is free of viral integration, exhibited pluripotent stem cell characteristics and able to differentiate to all embryonic lineages.
Authors
- Chuti Laowtammathron (ORCID: https://orcid.org/0000-0001-5912-6642)
- Nittaya Jiamvoraphong
- Thidathip Wongsurawat (ORCID: https://orcid.org/0000-0002-3659-2074)
- Surapol Issaragrisil (ORCID: https://orcid.org/0000-0002-8924-0646)
- Pattaraporn Nimsamer (ORCID: https://orcid.org/0000-0003-3741-7558)
- Chanchao Lorthongpanich (ORCID: https://orcid.org/0000-0003-2206-7065)
Institutions
- Siriraj Hospital (TH)
- Mahidol University (TH)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2025-09-23
- DOI
- https://doi.org/10.1016/j.scr.2025.103840
- Primary Topic
- T-cell and B-cell Immunology
- Type
- article
- Field-Weighted Citation Impact
- 0.00