BIOSYNTHESIS OF SILVER NANOPARTICLES FROM HIBISCUS FLOWERS AND CHECKING THEIR PROPERTIES

Nanotechnology is one of the emerging cutting-edge technologies in a variety of fields of science, including biomedical, industrial, and life science applications.Silver nanoparticles are one of the extensively studied nanomaterials due to their high stability and low chemical reactivity in comparison to other metals.They are commonly synthesized using chemicals that reduce metal ions into uncharged nanoparticles.In the last few decades, green synthesis methods have evolved to avoid the use of physical and chemical methods of synthesis.The natural biomolecules found in plants, such as enzymes, amino acids, alkaloids, and alcoholic compounds, are responsible for the formation of silver nanoparticles.The green synthesis of silver nanoparticles is an ecofriendly approach, which should be further explored for the potential of different plants to synthesize nanoparticles.This project focuses on the green synthesis of silver nanoparticles using the flowers of Hibiscus rosa-sinensis.The obtained silver nanoparticles were characterized by carrying out FTIR and UV-visible spectroscopy.The FTIR spectra showed deviations from the standard curve, which indicated the presence of impurities in the generated AgNPs.The silver nanoparticles were assessed for their antimicrobial activity, wastewater treating capacity, and haemolytic efficiency.The antimicrobial activity was found to be effective against Bacillus subtilis, Staphylococcus aureus and Aspergillus niger.The decrease in BOD levels indicates that high concentrations of AgNPs can be used to treat wastewater samples.The AgNPs were also found to be toxic at very high concentrations due to the haemolysis of RBCs, which indicated keeping a maximum concentration limit of their synthesis.In conclusion, the data obtained in the study highlights the role of biologically synthesized AgNPs as potential and promising nanomaterials in the field of life science.