GWAS-informed data integration and non-coding CRISPRi screen illuminate genetic etiology of bone mineral density
Our results provide a roadmap for how single-cell CRISPRi screens may be applied to the challenging task of resolving effector gene identities at all BMD GWAS loci. Extending our CRISPRi screening approach to other tissues could play a key role in fully elucidating the etiology of BMD.
Authors
- Alessandra Chesi (ORCID: https://orcid.org/0000-0002-0954-7446)
- Kurt D. Hankenson (ORCID: https://orcid.org/0000-0001-6361-143X)
- Eugene Katsevich (ORCID: https://orcid.org/0000-0003-0598-2050)
- Lacey J. Favazzo (ORCID: https://orcid.org/0000-0002-3203-0496)
- Matthew C. Pahl (ORCID: https://orcid.org/0000-0003-2969-6024)
- Cheryl L. Ackert‐Bicknell (ORCID: https://orcid.org/0000-0003-4253-4775)
- Benjamin Franklin Voight (ORCID: https://orcid.org/0000-0002-6205-9994)
- Michael J. Zuscik (ORCID: https://orcid.org/0000-0003-0461-8708)
- Andrew D. Wells (ORCID: https://orcid.org/0000-0002-3630-2145)
- Mitchell Conery (ORCID: https://orcid.org/0000-0001-7640-0797)
- David A. Villani (ORCID: https://orcid.org/0000-0002-4793-1625)
- Bao Khanh Trang (ORCID: https://orcid.org/0000-0002-9434-508X)
- Babette S. Zemel (ORCID: https://orcid.org/0000-0002-6164-7348)
- James A. Pippin (ORCID: https://orcid.org/0000-0002-6850-8164)
- Struan F.A. Grant (ORCID: https://orcid.org/0000-0003-2025-5302)
- Yadav Wagley (ORCID: https://orcid.org/0000-0002-1261-4267)
Institutions
- Children's Hospital of Philadelphia (US)
- University of Michigan (US)
- Translational Therapeutics (United States) (US)
- University of Colorado Anschutz Medical Campus (US)
- University of Pennsylvania (US)
Publication Details
- Journal
- Genome biology
- Published
- 2025-10-03
- DOI
- https://doi.org/10.1186/s13059-025-03802-4
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- AlloSource
- National Institute on Aging
- National Institute of Allergy and Infectious Diseases
- National Institute of Diabetes and Digestive and Kidney Diseases
- National Center for Advancing Translational Sciences