Neural growth patterns: how random and aligned fibers guide 3D cell organization and pseudospheroid formation
Fiber orientation significantly affected SH-SY5Y cell behaviour, including adhesion, formation of pseudospheroids, and differentiation marker expression under high-density conditions. Random and aligned fibers elicited distinct structural patterns and molecular responses, highlighting the importance of scaffold architecture in the rational design of neuroregenerative platforms. To our knowledge, this is the first study to describe scaffold-anchored neural pseudospheroids as a distinct model from conventional suspension spheroids.
Authors
- Aina‐Mari Lian (ORCID: https://orcid.org/0000-0001-7986-4845)
- Janne E. Reseland (ORCID: https://orcid.org/0000-0001-5743-4297)
- Jana Hlinková (ORCID: https://orcid.org/0000-0001-7261-1212)
- Anne Eriksson Agger (ORCID: https://orcid.org/0000-0002-9180-1297)
- Athina Samara (ORCID: https://orcid.org/0000-0001-6931-1972)
- Karolina Dziemidowicz (ORCID: https://orcid.org/0000-0001-5501-5646)
- Mathilde M. Ullrich
Institutions
- Karolinska University Hospital (SE)
- University of Oslo (NO)
- Karolinska Institutet (SE)
- Czech Academy of Sciences, Institute of Experimental Medicine (CZ)
- University College London (GB)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-10-03
- DOI
- https://doi.org/10.3389/fbioe.2025.1659965
- Citations
- 1
- Primary Topic
- Cellular Mechanics and Interactions
- Type
- article
- Field-Weighted Citation Impact
- 1.15
Funders
- University College London
- European Commission
- Akademie Věd České Republiky
- Horizon 2020 Framework Programme
- Engineering and Physical Sciences Research Council