Isolation and Enrichment of Outer Membrane-Derived Extracellular Vesicles from Bacteria

Source: Watson, D. C., et.al. Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria. J. Vis. Exp. (2021)This video demonstrates the isolation and concentration of extracellular vesicles (EVs) derived from the outer membrane of genetically engineered bacteria. The process involves culturing the bacteria to release EVs, followed by sequential centrifugation and filtration steps to remove cells and debris. Finally, ultrafiltration is used to concentrate the EVs for downstream analysis.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-09-26
DOI
https://doi.org/10.3791/23976
Primary Topic
Advanced Electron Microscopy Techniques and Applications
Type
article
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article

Isolation and Enrichment of Outer Membrane-Derived Extracellular Vesicles from Bacteria

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Journal of Visualized Experiments
Advanced Electron Microscopy Techniques and Applications
article

Isolation and Enrichment of Outer Membrane-Derived Extracellular Vesicles from Bacteria

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article en

Abstract

Source: Watson, D. C., et.al. Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria. J. Vis. Exp. (2021)This video demonstrates the isolation and concentration of extracellular vesicles (EVs) derived from the outer membrane of genetically engineered bacteria. The process involves culturing the bacteria to release EVs, followed by sequential centrifugation and filtration steps to remove cells and debris. Finally, ultrafiltration is used to concentrate the EVs for downstream analysis.

Journal of Visualized Experiments
Openalex Percentile: Top 88%
Advanced Electron Microscopy Techniques and Applications
DOISourceOpenAlex
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