Single-Cell Culture of Bacteria Within Giant Vesicles

Source: Morita, M., et al. Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles. J. Vis. Exp. (2019).This video demonstrates a method for culturing and observing individual bacterial cells encapsulated inside giant vesicles anchored to a supported lipid bilayer. The system enables stable, long-term imaging of single-cell growth dynamics in a confined, physiologically relevant microenvironment.