Imaging and Quantifying Swarm Avoidance Responses to Antibiotic Stress in <i>Pseudomonas aeruginosa</i>

Source: Bru, J., et.al. Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response. J. Vis. Exp. (2020)This video demonstrates how to visualize and measure swarm avoidance behavior in Pseudomonas aeruginosa using a swarming agar plate assay. It shows the setup for time-lapse imaging under controlled conditions and explains how antibiotic-induced stress signals redirect bacterial movement, which is quantified through image analysis.