A novel RP-UPLC method development and validation for quantification of flubendazole in bulk and pharmaceutical dosage form

RP-UPLC approach was used to produce a straightforward, accurate, and exact method for estimating flubendazole.For chromatography, stationary phase C18 (4.6 x50 mm,5 m) was utilized.Acetonitrile and HPLC-grade water in 700:300 ratio was used as the mobile phase.The flow rate was kept at 0.45 ml/min, the detection wavelength was set at 230nm.six standard injections was used to study the system suitability characteristics, and the finding fell well within the acceptable range.The r2 value was 1.0000 after a linearity analysis was conducted at 80% to 120%level.The results showed that the precision was 0.66 for intermediate precision and 0.73 for repeatability.Respectively the LOD and LOQ values was found to be0.002 g/ml and 0.006 g/ml.