Antiviral Activity of Origanum vulgare ssp. hirtum Essential Oil-Loaded Polymeric Micelles

<b>Background</b>: Encapsulating essential oils in polymer-based nanocarriers can improve their stability, solubility, and bioavailability, while maintaining the biological activity of the oil's active ingredients. In this contribution, we investigated the antiviral activity of Oregano Essential Oil (OEO) in its pure form and encapsulated into nanosized polymeric micelles, based on a poly(ethylene oxide)-block-poly(ε-caprolactone) diblock copolymer. <b>Methods</b>: The effect of encapsulation was evaluated using three structurally different viruses: herpes simplex virus type 1 (HSV-1) (DNA-enveloped virus), human coronavirus (HCoV OC-43) (RNA-enveloped virus), and feline calicivirus (FCV) (RNA-naked virus). The effect on the viral replicative cycle was determined using the cytopathic effect inhibition (CPE) test. Inhibition of the viral adsorption step, virucidal activity, and protective effect on healthy cells were assessed using the final dilution method and were determined as Δlg compared to the untreated viral control. <b>Results</b>: In both studied forms (pure and nanoformulated), OEO had no significant effect on viral replication. In the remaining antiviral experiments, the oil embedded into nanocarriers showed a slightly stronger effect than the pure oil. When the oil was directly applied to extracellular virions, viral titers were significantly reduced for all three viruses, with the effect being strongest for HSV-1 and FCV (Δlg = 3.5). A distinct effect was also observed on the viral adsorption stage, with the effect being most significant for HSV-1 (Δlg = 3.0). <b>Conclusions</b>: Pretreatment of healthy cells with the nanoformulated OEO significantly protected them from viral infection, with the greatest reduction in viral titer for HCoV OC-43.