<i>Desulfosporosinus shakirovi</i> sp. nov., a sulfate-reducing bacterium with ability to petroleum hydrocarbons degradation

A new sulfate-reducing strain SRJS8T with a high capacity to biodegradate petroleum hydrocarbons has been isolated from the bottom sediments of the northern part of the Sea of Japan. The cells were gram-positive, single-spore-forming motile rods 0.4–0.5 × 2.0–5.0 µm in size. Strain SRJS8T grew at the temperature range from 6°C to 30°C (optimum at 25°C), pH 6.3–7.7 (optimum 7.3), and NaCl concentrations ranging from 0 to 20g/L (optimum 2 g/L). Strain SRJS8T used butanol, glycerol, methanol, ethanol, butyrate, lactate, pyruvate, formate, yeast extract, H2/CO2, crude oil as electron donors and carbon source in the presence of sulfate. SRJS8T used sulfate, sulfite, thiosulphate, elemental sulphur, fumarate and Fe(III) as electron acceptors with lactate presence. The closest relative of the SRJS8T was Desulfosporosinus lacus STP12T with 98.49% similarity. The complete genome of strain SRJS8T (5.43 Mb) was sequenced. DNA–DNA homology of the SRJS8T and D. lacus was 57.4%, and the ANI value was 93.69%. The genomic DNA G + C content was 42.08%. In accordance to the obtained data, SRJS8T is a novel species within the Desulfosporosinus genus, for which the name Desulfosporosinus shakirovi sp. noV. is proposed. The type strain is SRJS8T (= VKM B-3489T, = JCM 39189T).