ABP1/ABL3-TMK1 cell-surface auxin signaling targets PIN2-mediated auxin fluxes for root gravitropism
Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a prerequisite for (1) self-organizing processes, including vascular tissue formation, and (2) directional growth responses such as gravitropism. Here, we identify a mechanism by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface AUXIN-BINDING PROTEIN1 (ABP1)-TRANSMEMBRANE KINASE 1 (TMK1) receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE 3 (ABL3) auxin receptor. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably for other self-organizing developmental phenomena.
Authors
- Yongfan Yu
- Chen Meng (ORCID: https://orcid.org/0000-0002-5968-6719)
- Gergely Molnár (ORCID: https://orcid.org/0000-0001-6205-4723)
- Lukas Hoermayer (ORCID: https://orcid.org/0000-0001-8295-2926)
- Lanxin Li (ORCID: https://orcid.org/0000-0002-5607-272X)
- Yvon Jaillais (ORCID: https://orcid.org/0000-0003-4923-883X)
- María Mar Marquès‐Bueno (ORCID: https://orcid.org/0000-0003-3178-1922)
- Jakub Hajný (ORCID: https://orcid.org/0000-0003-2140-7195)
- Inge Verstraeten (ORCID: https://orcid.org/0000-0001-7241-2328)
- Lesia Rodríguez (ORCID: https://orcid.org/0000-0002-7244-7237)
- Aline Monzer (ORCID: https://orcid.org/0000-0002-1951-9748)
- Marek Randuch (ORCID: https://orcid.org/0000-0002-8108-0158)
- Minxia Zou (ORCID: https://orcid.org/0009-0007-8073-8644)
- Caterina Giannini (ORCID: https://orcid.org/0000-0003-2757-6782)
- Shutang Tan (ORCID: https://orcid.org/0000-0002-0471-8285)
- Zuzana Gelová (ORCID: https://orcid.org/0000-0003-4783-1752)
- Ivan Kulich (ORCID: https://orcid.org/0000-0002-0458-6470)
- Lukáš Fiedler (ORCID: https://orcid.org/0000-0002-2454-4665)
- Dmitrii Vladimirtsev
- Zainab Quddoos
Institutions
- Université Claude Bernard Lyon 1 (FR)
- École Normale Supérieure de Lyon (FR)
- Centre National de la Recherche Scientifique (FR)
- Institute of Science and Technology Austria (AT)
- Hefei National Center for Physical Sciences at Nanoscale (CN)
- Laboratoire Reproduction et Développement des Plantes (FR)
- Czech Academy of Sciences, Institute of Experimental Botany (CZ)
- Palacký University Olomouc (CZ)
Publication Details
- Journal
- Cell
- Published
- 2025-10-01
- DOI
- https://doi.org/10.1016/j.cell.2025.08.026
- Citations
- 15
- Primary Topic
- Plant Molecular Biology Research
- Type
- article
- Field-Weighted Citation Impact
- 26.25