A universal, high-quality, and high-yield DNA purification method for mycobacteria, including <i>Mycobacterium tuberculosis</i> : large-scale assessment of the chloroform-bead method

Mycobacterial genomics is crucial for understanding pathogenesis and drug resistance; however, DNA extraction remains a significant challenge because of its unique cell wall. Traditional methods rely on enzymatic treatments, resulting in complex and time-consuming protocols with variable results. The chloroform-bead method introduces a paradigm shift by chemically and mechanically disrupting the mycolic acid layer and eliminating the need for enzymatic treatment. This standardized approach ensures consistent, high-quality DNA extraction across diverse mycobacterial species, thereby enhancing research capabilities and clinical applications.

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Publication Details

Journal
Microbiology Spectrum
Published
2025-10-02
DOI
https://doi.org/10.1128/spectrum.00765-25
Citations
3
Primary Topic
Mycobacterium research and diagnosis
Type
article
Field-Weighted Citation Impact
4.06
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article

A universal, high-quality, and high-yield DNA purification method for mycobacteria, including Mycobacterium tuberculosis : large-scale assessment of the chloroform-bead method

Yoshiko Shimomura, F Tani, Kentaro Arikawa, Masayuki Yajima et al.
3 citations
Microbiology Spectrum
Mycobacterium research and diagnosis
4.06
article

A universal, high-quality, and high-yield DNA purification method for mycobacteria, including Mycobacterium tuberculosis : large-scale assessment of the chloroform-bead method

Yoshiko Shimomura, F Tani, Kentaro Arikawa, Masayuki Yajima, Atsuko Tanouchi, Yuta Morishige, Satoshi Mitarai, Akito Mizokoshi, Akiko Takaki, Yusuke Yodotani, Saori Ueda, Hitoshi Otsuka, Makiko Hosoya, Minami Nagai, Keiko Fujiwara, Kaori Yamamoto, Aki Tamaru, Yoshiro Murase, Nozomi Kamitaka, Hiroki Hayashi, Yohei Takahashi, Yuko Yamaguchi, Shinichiro Shibata, Kaori Shimmen
article en
3 citations

Abstract

Mycobacterial genomics is crucial for understanding pathogenesis and drug resistance; however, DNA extraction remains a significant challenge because of its unique cell wall. Traditional methods rely on enzymatic treatments, resulting in complex and time-consuming protocols with variable results. The chloroform-bead method introduces a paradigm shift by chemically and mechanically disrupting the mycolic acid layer and eliminating the need for enzymatic treatment. This standardized approach ensures consistent, high-quality DNA extraction across diverse mycobacterial species, thereby enhancing research capabilities and clinical applications.

Microbiology SpectrumVol. 13(11)
Himeji University (JP), Institute of Public Health (AL), Fukuoka Institute of Health and Environmental Sciences (JP), Aomori Prefectural School Education Center (JP), Japan Anti Tuberculosis Association (JP), Osaka Prefectural Institute of Public Health (JP), Shimane Prefectural Institute of Public Health and Environmental Sciences (JP), Aomori Prefectural Industrial Technology Center (JP), Yamagata Prefectural Institute of Public Health (JP), Kyoto Prefectural Institute of Public Health and Environment (JP), Aichi Prefectural Government (JP), Nagasaki University (JP), Institute of Public Health Zadar (HR)
Openalex Percentile: Top 5%
Mycobacterium research and diagnosis
4.06
DOIOpen Access PDFOpenAlex
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