IGFBP2 Mediates Human iPSC-Cardiomyocyte Proliferation in a Cellular Contact-Dependent Manner
Our findings demonstrate that cell-cell contact inhibits hiPSC-CM proliferation through adherens junction formation, sarcomeric assembly, and reduced IGFBP2 secretion. Importantly, exogenous supplementation of IGFBP2 can overcome cell contact-mediated inhibition of hiPSC-CM proliferation and facilitate the growth of 3-dimensional cardiac tissue. These insights provide valuable implications for advancing cardiac tissue engineering and regenerative therapies.
Authors
- Yongwon Kang
- David T. Paik (ORCID: https://orcid.org/0000-0002-7830-312X)
- Soah Lee (ORCID: https://orcid.org/0000-0001-7613-592X)
- William R. Goodyer (ORCID: https://orcid.org/0000-0002-3252-1180)
- Francisco X. Galdos (ORCID: https://orcid.org/0000-0002-7985-4521)
- Seung‐Woo Cho (ORCID: https://orcid.org/0000-0001-8058-332X)
- Jihye Yun (ORCID: https://orcid.org/0009-0004-4270-3107)
- Nadjet Belbachir (ORCID: https://orcid.org/0000-0002-6993-8326)
- Sungjin Min (ORCID: https://orcid.org/0000-0002-5041-8638)
- Joseph C. Wu (ORCID: https://orcid.org/0000-0002-6068-8041)
- James E. Hudson (ORCID: https://orcid.org/0000-0003-0832-9356)
- Sean J. Humphrey (ORCID: https://orcid.org/0000-0002-2666-9744)
- Boyoung Kim (ORCID: https://orcid.org/0009-0000-7250-8565)
- Alessandra Moretti (ORCID: https://orcid.org/0000-0001-5782-7832)
- Jan W. Buikema (ORCID: https://orcid.org/0000-0001-9313-0602)
- Sean M. Wu (ORCID: https://orcid.org/0000-0002-0000-3821)
- Paul V. Heinrich (ORCID: https://orcid.org/0000-0003-4899-8343)
- Harley Robinson (ORCID: https://orcid.org/0000-0002-9374-5532)
- Daniel Lee
- Jaecheol Lee (ORCID: https://orcid.org/0000-0002-2070-7463)
Institutions
- Royal Children's Hospital (AU)
- Queensland University of Technology (AU)
- Yonsei University (KR)
- California Institute for Regenerative Medicine (US)
- QIMR Berghofer Medical Research Institute (AU)
- Cardiovascular Institute of the South (US)
- TUM Klinikum (DE)
- Yale University (US)
- Institute for Stem Cell Biology and Regenerative Medicine (IN)
- Institute for Basic Science (KR)
- German Centre for Cardiovascular Research (DE)
- Deutsches Herzzentrum München (DE)
- Amsterdam University Medical Centers (NL)
- Sungkyunkwan University (KR)
- Amsterdam UMC Location Vrije Universiteit Amsterdam (NL)
- Stanford University (US)
Publication Details
- Journal
- Circulation Research
- Published
- 2025-10-01
- DOI
- https://doi.org/10.1161/circresaha.125.326522
- Primary Topic
- Cardiomyopathy and Myosin Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00