Protective Effect of Aromatic Plant Essential Oil Administration on Brain Tissue of PTZ-Treated and Non-Treated Mice

Epilepsy manifests as recurrent spontaneous seizures associated with irregular brain activity. Recognizing the limitations of conventional antiepileptic treatments, we explored the therapeutic potential of essential oils (EOs) derived from Greek aromatic plants (<i>Mentha pulegium</i>, <i>Mentha spicata</i> wild, <i>Mentha piperita</i>, <i>Lavandula angustifolia</i> and <i>Origanum Dictamnus</i>). Specifically, we explored their radical scavenging capacity (DPPH), as well as their antioxidant (AOP and MDA levels) and neuroprotective effect in a PTZ-induced epilepsy Balb/c mice model (animals were pretreated with EOs prior to PTZ treatment). Our results indicated that <i>Mentha piperita</i> emerges as the most promising EO, demonstrating strong antioxidant activity and the highest radical scavenging ability (IC₅₀ = 1.9 mg/mL). <i>Mentha pulegium</i> also exhibited considerable antioxidant potential, demonstrating the strongest effect in the AOP assay when administered prior to PTZ treatment. Furthermore, <i>Origanum dictamnus</i> exhibited the strongest potential to attenuate MDA formation in the presence of PTZ. Finally, immunohistochemistry indicated a trend of neuronal preservation in animals pretreated with EOs prior to PTZ, with <i>Mentha piperita</i> demonstrating the most significant effect. Based on these findings, we suggest that certain EOs possess significant antioxidant and neuroprotective properties. Further research is warranted to validate these results and elucidate the active ingredients responsible for the observed properties.