Development of CRISPR/Cas9-mediated CD16b-/- and CD32a-/- promyelocytic cell lines to study FcγR signaling in human neutrophils
Both CD16b<sup>-/-</sup> and CD32a<sup>-/-</sup> HL-60-derived clones maintained neutrophilic differentiation and phagocytic capacity but displayed impaired FcγR-mediated ROS production and SYK phosphorylation, with more pronounced defects in CD16b<sup>-/-</sup> cells. Cytokine production was altered in both lines, with CD16b<sup>-/-</sup> cells producing less IL-6 and IL-1β, and CD32a<sup>-/-</sup> cells producing less TNF-α and IL-10. This model provides new insights into the distinct roles of CD16b and CD32a in neutrophil activation and immune responses.
Authors
- Maria Rodríguez‐Gonzalez
- José Antonio Cruz-Cárdenas
- Marion E. G. Brunck (ORCID: https://orcid.org/0000-0001-8994-8276)
- Alejandra López-Arredondo (ORCID: https://orcid.org/0000-0003-3557-5122)
- Laura A. Palomares (ORCID: https://orcid.org/0000-0002-2811-8102)
- Jorge Andrés Cázares-Preciado (ORCID: https://orcid.org/0000-0003-2661-584X)
Institutions
- Universidad Autónoma de la Ciudad de México (MX)
- Universidad Autónoma del Estado de Morelos (MX)
- Universidad Nacional Autónoma de México (MX)
- Tecnológico de Monterrey (MX)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-10-02
- DOI
- https://doi.org/10.3389/fimmu.2025.1633609
- Primary Topic
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00