B-002 Clinical Evaluation of Portable and Maintenance-Free Blood Gas and Electrolyte Analysis Systems for Veterinary Clinics

Abstract Background Blood gas and electrolyte analysis has become an essential diagnostic tool for modern veterinary clinics. Traditional blood gas parameters (pH, pCO2, pO2) are essential for assessing acid-base imbalances, hypoxia, and carbon dioxide retention in critically ill animals. Additionally, integration of electrolytes and biochemical parameters enhances the assessment of trauma, respiratory, metabolic, and endocrine disorders. To address the diverse needs of veterinary point-of-care testing (POCT), Eaglenos has developed several types of integrated blood gas and electrolyte test cartridges. This pilot study compares two representative models: 1) EG10+ measures 10 parameters including blood gases and lactate; 2) L5CV measures pH, pCO2, Na+, K+, and Cl-, against the benchmark devices for performance validation. Methods Approximately 300 µL of fresh canine or feline venous blood samples were collected in lithium heparin-coated tubes at veterinary hospitals. Samples were equally divided and analyzed using both Eaglenos and the benchmark devices for comparison. For comparison of pH, pCO2, pO2, and lactate, we compared 1) Abbott i-STAT CG4+ vs. Eaglenos EG10+ (n=28); 2) Radiometer ABL80 vs. Eaglenos EG10+ (n=17). For pH, pCO2, Na+, K+, and Cl-, we compared the Radiometer ABL80 and Eaglenos L5CV (n=6). All comparisons were evaluated using correlation analysis, Passing-Bablok regression, and compliance with total allowable error (TEa) in American Society for Veterinary Clinical Pathology (ASVCP) guidelines. Results For the performance assessment of pH, pCO2, pO2, and lactate, the Pearson correlation coefficients of both pairs were greater than 0.95. In particular, Eaglenos EG10+ demonstrated excellent correlation and consistency compared with Radiometer. For the performance assessment of pH, pCO2, Na+, K+, and Cl-, the Pearson coefficients were greater than 0.95 (with the exception of pH, which is 0.947). All deviations remained within the acceptable TEa limits specified in ASVCP guidelines. Additionally, Passing-Bablok regression analysis showed statistically significant regression, with the intercept close to 0 and the slope near 1, indicating strong agreement between the compared methods. Conclusion Eaglenos has developed integrated, single-use cartridge based blood gas and electrolyte analysis systems for veterinary POCT, demonstrating accuracy comparable to benchmark products. Preliminary studies have confirmed strong analytical performance in veterinary clinical sample analysis. EG10+ allows for comprehensive physiological screening, assessing multiple organ systems within a single test; L5CV is designed for blood gas and electrolyte balance assessment, with a particular focus on acute gastrointestinal disturbances frequently seen in veterinary hospitals. Additionally, the device software provides automated calculations for key parameters such as TCO2, HCO3-, AG, and BE, essential for differentiating acid-base disorder types. Additional advantages include software-assisted interpretation of abnormal values, diagnostic support, and durable test cartridges that remain stable during transport and long-term storage at room temperature. These features align with clinical needs and optimize the efficiency of veterinary diagnostics.