An activity-based probe library for identifying promiscuous amide hydrolases
A fluorogenic substrate library was developed to detect amide hydrolase activity in soil-derived chitin-degrading bacteria. Hit compounds were converted into pull-down probes for chemoproteomic enrichment, identifying previously unannotated proteins now linked to putative hydrolases. This approach prioritizes candidate hydrolases for further experimental validation with potential applications in the environment, biomanufacturing, and medicine.
Authors
- Stephen Callister (ORCID: https://orcid.org/0000-0003-1785-2755)
- Chathuri J. Kombala (ORCID: https://orcid.org/0000-0002-3847-8769)
- Jared O. Kroll (ORCID: https://orcid.org/0000-0001-9066-0579)
- Irlanda N Medrano
- Sankarganesh Krishnamoorthy (ORCID: https://orcid.org/0000-0001-6264-8791)
- Kristoffer Brandvold (ORCID: https://orcid.org/0000-0001-5970-879X)
- Natalie Sadler (ORCID: https://orcid.org/0000-0003-1161-5687)
- Erin Bredeweg
- Lucas Webber
Institutions
- Pacific Northwest National Laboratory (US)
- Environmental Molecular Sciences Laboratory (US)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc04162g
- Primary Topic
- Chemical Synthesis and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Energy
- Battelle
- Office of Science
- Biological and Environmental Research
- Pacific Northwest National Laboratory