In Vitro Controlling Of Wound Pathogens Using Andrographis Paniculata Stem Mediated Selenium Nanoparticles

Introduction: Wound infections, caused by microorganisms such as Staphylococcus aureus and Escherichia coli, impede healing and damage tissue. Developing eco-friendly nanoparticle synthesis methods is crucial, with selenium nanoparticles (SeNPs) showing promise in biomedicine due to their low toxicity and high biocompatibility. SeNPs, produced by microorganisms or chemical methods, exhibit significant antioxidant and anti-inflammatory properties. The medicinal herb Andrographis paniculata, rich in polyphenols, flavonoids, and diterpenoids, has been traditionally used to treat various infections and inflammatory conditions, enhancing immune responses and offering potential therapeutic benefits. Aim: In vitro controlling of wound pathogens using Andrographis paniculata stem mediated selenium nanoparticles has been evaluated Methods: Preparation: Andrographis paniculata stem extract was mixed with sodium selenite solution to synthesize SeNPs.The agar well diffusion method was carried out against Staphylococcus aureus, Pseudomonas sp, Escherichia coli. Results: Andrographis paniculata stem-mediated selenium nanoparticles (SeNPs) show significant antibacterial activity against Staphylococcus aureus, with effectiveness increasing at higher concentrations. SeNPs also exhibit moderate, consistent activity against Pseudomonas sp. and Escherichia coli. Further research could explore their mechanism, stability, and potential in antimicrobial therapies. Conclusion: The SeNPs synthesized from the stem extract of Andrographis paniculata showed excellent antibacterial potential; hence further studies are needed to assess the efficacy and safety for dental uses.