High Performance Liquid Chromatographic Analysis of Sugars in Honeys from Western Ghats, Karnataka

In the present study, pollen, physical and sugar analysis of five uniflora land five multifloral honeys were carried out. The main floral sources identified were coffee, coriander, neem, niger and acacia. Their sugar profile was analyzed by means of High performance liquid chromatography with refractive index detector. This method enables the determination of five main sugars of honey such as glucose, fructose, galactose, sucrose, and maltose. Sugar concentration of different types of honeys were found variable and the result obtained showed that the predominant sugar of the 5 investigated unifloral honeys and 5 multifloral honeys was fructose followed by glucose and maltose. Galactose and sucrose were present in low amount in all the samples.