Effect of the nitrogen monoxide synthesis donor and inhibitor on the stress metabolites level of basidiomycetes under abiotic stress conditions

Nitrogen monoxide in fungi participates in the processes of reproduction, pathogenesis and adaptation to environmental conditions. Substances that affect the NO content in fungal cultures have an effect on growth processes and the synthesis of other metabolites. The effect of a donor and an inhibitor of NO synthesis on the production of stress metabolites of basidiomycetes Lentinus edodes and Grifola frondosa under abiotic stress conditions (high- and low-temperature shock, lack of carbon and/or nitrogen in the nutrient medium) was studied. Under all the conditions studied, the introduction of L-NAME (NO synthesis inhibitor) into the medium stimulated, and SNP (NO donor) suppressed the growth of fungal mycelium. At the same time, the content of the protective stress metabolites trehalose, mannitol and proline in both basidiomycetes increased to 70% in media with SNP and decreased to 65% in media with L-NAME. The most pronounced effect of SNP and L-NAME on the growth of fungal cultures and their accumulation of protective compounds turned out to be under the influence of temperature stress.