REGULARITIES OF RETENTION OF ALCOHOLS, KETONES AND SUGARS ON A SULPHONIC ACID CATION-EXCHANGER IN H, Ca- AND La-FORMS

The chromatographic retention of alcohols, ketones, alditols and carbohydrates on a cation exchange column (200 × 4.6 mm I.D., Sevko AA) packed with sulfonated poly(styrene-divinylbenzene) with a crosslinking degree of 10 % and a particle diameter of 7 μm saturated with various counterions (H+, Ca2+ and La3+) is studied in the ligand exchange chromatography variant. The sorbent in the Ca2+ form shows higher retention times and selectivity in the separation of carbohydrates and ethylene glycols. The sorbent in the La3+ form provides strong retention and better selectivity for the separation of alditols. The possibility of using this column for determining carbohydrates in food products (juice, honey) using refractometric detection is shown.