New micellar polymers as polyfunctional modifiers of electrophoretic systems

New modifiers of electrophoretic systems – cationic polyelectrolytes with micellar properties poly-11-acryloyloyloxyundecyl-N-methylpiperidinium bromide (pAUMP-Br), poly-11-acryloyloxyundecylpyridinium bromide, poly-11-acryloyloxyundecyl-1,4-diazobicyclo[2. 2.2.2.2]octan-1-ium bromide and a chiral copolymer based on acylated quinine and N-(11-acryloyloyloyloxyundecyl)-N-methylpiperidinium bromide (pAUMP-AQin). The electrophoretic capabilities of these polymers were studied in the separation of model mixtures of biologically active substances: steroid hormones, biogenic amines and amino acids in the modes of micellar electrokinetic chromatography and capillary electrochromatography. It was found that new polyelectrolytes are polyfunctional modifiers of quartz capillary walls and background electrolyte and allow to realize different modes of electrophoretic separation of biologically active substances, indirect detection in the presence of chromophoric group in the polymer composition, generate anodic electroosmotic flow, influence the efficiency and selectivity of analytes separation. Field-amplified sample stacking was performed on a quartz capillary modified with pAUMP-Br, providing a 15-fold concentration of biogenic amines and lowering the detection limits. The chiral labeled pAUMP-AQin copolymer was found to facilitate the separation of tryptophan enantiomers.