Membrane methods for the production of horseradish peroxidase conjugates for the detection of viral infections

An approach to the use of membrane filtration in the development of techniques for the preparation of horseradish peroxidase-based conjugates for the determination of viral infections has been proposed. It is shown that this approach is much simpler, faster and cheaper than the chromatographic approach. There are practically no losses of conjugates during purification of components. A technique for membrane purification of horseradish peroxidase and its analysis by capillary electrophoresis is proposed. The technique also allows preparation of horseradish peroxidase and virus antigen conjugate for use in enzyme-linked immunosorbent assay (ELISA) for detection of class M antibodies to the virus (in modification of M antibody capture). The practice of using a variety of diagnostic kits prepared using this approach, for example in ELISA test systems, for the diagnosis of diseases caused by West Nile virus, Crimean-Congo haemorrhagic fever, tick-borne encephalitis, etc. is described.