Determination of residual amounts of beta-adrenostimulants in muscle tissue and liver by dispersion purification chromatography-mass spectrometry

A selective technique for the determination of 20 beta-adrenostimulants in muscle tissue and liver by chromatography-mass spectrometry has been developed. The limit of quantitative determination of the technique is 0.1-0.25 (0.5) µg/kg (depending on the compound to be determined). The technique is based on hydrolysis of research objects followed by extraction of the determined compounds with acetic acid acetonitrile, obtaining the organic fraction by salting, its purification on aluminum oxide layer and dispersive purification with C18, further purification by liquid-liquid extraction with hexane. Data on promising ion-transitions of some determined compounds are given; detection parameters of bambuterol, formoterol, phenylethanolamine A are established. Data on optimization of hydrolysis, extraction and purification of extracts are given. Information on stage-by-stage losses of the determined compounds is given; data on the analysis of reference material are obtained. The validation of the developed method shows that the relative expanded uncertainty ranges from 7 to 24 %.