HPLC-MS/MS-determination of antihistamines cyproheptadine, diphenhydramine and tripelenamine in foods and feeds

The method of determination of antihistamine substances cyproheptadine, diphenhydramine and tripelenamine in food and feed by high-performance liquid chromatography-tandem mass spectrometry was proposed. When developing the method, its versatility in terms of reagents and equipment used in the analysis of matrices of different types – meat, offal (liver), eggs, milk, honey, butter, as well as mixed fodder – was considered as the main aspect. A combination of solvents was used for the isolation of analytes: acetonitrile, water and hexane (3 ml of each), the sequence of solvent addition depended on the matrix, the rest of the sample preparation steps were unified. The limits of detection and determination of antihistamines in the above objects were 0.012-0.3 and 0.04-1 μg/kg, respectively. The relative standard deviation of the analytical results does not exceed 17 %. The relative error of determination of substances in most cases does not exceed 15 %.