Electrochemical-based In situ Fingerprinting of Bioactive Phlorotannins from Isolates of Marine Algae

Brown algal extracts contain rich amount of phloroglucinol-based polyphenolic compounds called phlorotannins (PTs) which have diverse functional properties. Quality control and assurance of mixed polyphenols particularly from marine algal extracts is a complex process. Electrochemical fingerprinting is recently emerging as potential alternative for structural elucidation of natural products. In this work, we attempt to electrochemically fingerprint the isolates of marine algae, Sargassum wightii. Cyclic voltammograms of both extracts enabled irreversible oxidation peaks associated to phloroglucinol structure, following 1e<sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">−</sup>/1H<sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+</sup> transfer under phosphate buffered saline electrolyte. The optical activity of the electrophoretically separated phloroglucinol constitute negligible pigment traces than solid-liquid extracted sample. Drop-casted and electrophoretically-modified phloroglucinol solid surface shows their promising application for optical / electrochemical sensor application.