Development of a Protocol for Transformation of RG

Lactic acid bacteria, traditionally used in the food industry, are considered as a platform for the creation of recombinant strains for the expression and delivery of protectively significant antigens and other biologically active molecules to the gastrointestinal tract of farm animals and humans. One of the obstacles to obtaining recombinant strains of lactic acid bacteria is their low efficiency of plasmid DNA transformation. The probiotic strain RG, safe and able to overcome the acidic barrier of the stomach, was chosen as the object of research. The effect of growth conditions, preparation of competent cells and the electroporation mode on the efficiency of RG transformation with the pTRKH2 vector was studied, as a result of which a protocol for efficient transformation of RG with recombinant DNA was created.