Inactivation of the gene responsible for the synthesis of gluconic acid in the genome of <i>Pantoea brenneri</i>

Abstract. To study the contribution of the glucose dehydrogenase (gcd) gene product to the development of ISR priming in plants, a strain of Pantoea brenneri 3.2 with a deletion of the gcd gene was obtained. Using the Lambda Red (λ Red) phage recombination system, we obtained a marker-free mutant strain of P. brenneri 3.2 Δgcd. Inactivation of the gcd glucose dehydrogenase gene resulted in a 2.5-fold decrease in the strain’s ability to solubilize tricalcium phosphate on the solid nutrient medium NBRIP.