Genomic and functional analysis of a representative of the rare genus <i>Krasilnikoviella</i> ‒ Strain YrIIa5 with multiple antibiotic resistance

The increasing interest in the so-called “rare genera” of actinomycetes is associated with insufficiently studied of their potential as producers of valuable metabolites, primarily antibiotics. The use of selective methods made it possible to isolate the YrIIa5 strain from the soil collected near the “Dutch Fortress” (Rostov, Yaroslavl Region). Phylogenetic analysis based on the similarity of the 16S rRNA genes and five housekeeping genes (MLSA) allowed us to assign the isolate to the genus Krasilnikoviella. Genome analysis revealed many genes responsible for resistance to a wide range of antibiotics, which was confirmed by studying the strain for sensitivity to antimicrobial compounds using the agar diffusion method.